Technology

Traditionally, the fluorescence lifetime decay curve has been reduced to a
single number, the fluorescence lifetime. This is unfortunate because the
waveform contains information which is discarded during this process. By
automatically comparing waveforms mathmatically with our proprietary
software we are able to use the information inherent in the waveform to
reduce many sources of noise and generate a confidence factor for each
sample. The net result is we can better distinguish hits from noise and
have confidence that hits are real and not from some artifact.

Direct Wavefrom Recording is our name for our method of capturing and
comparing fluorescence lifetime waveforms generated by a pulsed laser
directed onto the sample of interest. Utilizing ultra high speed digitizers,
timing electronics, and proprietary software we record the entire waveform
generated from every pulse of lasers that pulse at up to 10,000 times per
second. We therefore acquire as many as 10,000 waveforms per second.
This means that even at read speeds of 25 wells per second in a plate
reader format we can average hundreds of waveforms per well, resulting in
exceptional signal to noise.

If you see a change in fluoresence intensity or emission spectrum there is usually a corresponding change in the fluorescence lifetime
waveform. Even a small change in this waveform yields excellent results with Direct Waveform Recording. Refer to our applications or
contact us regarding your specific needs.

With Direct Waveform Recording, the nanoseconds duration
fluorescence lifetime decay curve is captured thousands of times
per second. The average of waveforms collected in a few
milliseconds is extremely reproducible leading to highest quality
data even at high read speeds.